Frog-derived antibacterial peptide Temporin-B7K11K as well as expression method and application thereof
The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Tem...
Saved in:
Main Authors | , , , , |
---|---|
Format | Patent |
Language | Chinese English |
Published |
12.09.2023
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Temporin-B7K11K provided by the invention is LLPIVGKLLKKLL (SEQ ID NO: 1), a codon for coding the frog-derived antibacterial peptide Temporin-B7K11K is optimized, a secretory expression vector pPIC9K and a Mut + type host bacterium Pichia pastoris GS115 are selected, a gene recombination strain for efficiently expressing the antibacterial peptide Temporin-B7K11K is constructed, fermentation conditions of recombinant pichia pastoris are optimized, and secretory expression of the antibacterial peptide Temporin-B7K11K is carried out. The antibacterial peptide is good in stability and high in expression quantity, has broad-spectrum antibacterial activity and low hemolytic activity, particularly has a relatively high killi |
---|---|
AbstractList | The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Temporin-B7K11K provided by the invention is LLPIVGKLLKKLL (SEQ ID NO: 1), a codon for coding the frog-derived antibacterial peptide Temporin-B7K11K is optimized, a secretory expression vector pPIC9K and a Mut + type host bacterium Pichia pastoris GS115 are selected, a gene recombination strain for efficiently expressing the antibacterial peptide Temporin-B7K11K is constructed, fermentation conditions of recombinant pichia pastoris are optimized, and secretory expression of the antibacterial peptide Temporin-B7K11K is carried out. The antibacterial peptide is good in stability and high in expression quantity, has broad-spectrum antibacterial activity and low hemolytic activity, particularly has a relatively high killi |
Author | SUN HONGHAO LYU FUJUN SUN XIAOHAN GAO GAILIN ZHANG JIAQING |
Author_xml | – fullname: SUN HONGHAO – fullname: GAO GAILIN – fullname: SUN XIAOHAN – fullname: LYU FUJUN – fullname: ZHANG JIAQING |
BookMark | eNqNjE0KwjAUhLPQhX93iAcoGAvtWotFKLjqvsR22gbSvEcS1OPbggdwMXzMxzBbsXLksBFD6WlIOnjzQie1i-ap2zhXbSWDo-kga0xM3rjkmldKVVIH-Ya1C_FhjxAMOTkhjrRczGG2ptVx0XGEB_V7se61DTj8uBPH8lYX9wRMDQLrFg6xKR5KZXmq1Pl0Sf_ZfAGvQkEB |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences |
DocumentTitleAlternate | 一种蛙源抗菌肽Temporin-B7K11K及其表达方法和应用 |
ExternalDocumentID | CN116731120A |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_CN116731120A3 |
IEDL.DBID | EVB |
IngestDate | Fri Jul 19 12:58:56 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | Chinese English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_CN116731120A3 |
Notes | Application Number: CN202310874971 |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230912&DB=EPODOC&CC=CN&NR=116731120A |
ParticipantIDs | epo_espacenet_CN116731120A |
PublicationCentury | 2000 |
PublicationDate | 20230912 |
PublicationDateYYYYMMDD | 2023-09-12 |
PublicationDate_xml | – month: 09 year: 2023 text: 20230912 day: 12 |
PublicationDecade | 2020 |
PublicationYear | 2023 |
RelatedCompanies | LIAONING POWER LIGHT AGRICULTURE AND ANIMAL HUSBANDRY INDUSTRIAL CO., LTD |
RelatedCompanies_xml | – name: LIAONING POWER LIGHT AGRICULTURE AND ANIMAL HUSBANDRY INDUSTRIAL CO., LTD |
Score | 3.6265037 |
Snippet | The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
Title | Frog-derived antibacterial peptide Temporin-B7K11K as well as expression method and application thereof |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230912&DB=EPODOC&locale=&CC=CN&NR=116731120A |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dT8IwEL8gGvVNUaP4kZqYvTWyjbHtYTHSsRAJgxg0vJGOdYAxG4H5Ef96r2WIL_rQrOmSy3rt7a69u98B3NiiYVljbtC6I0xaT2xOI3OMC2I4VlxLEtSKMsG5GzbaT_WHoTUswcs6F0bhhH4ocESUqDHKe67-1_PNJZavYiuXt9EMh7K7YOD5WnE6Rnva1Q3Nb3qtfs_vMY0xj4Va-OhJd4OJtkXtfgu2pRktcfZbz02ZlTL_rVKCA9jpI7U0P4TS17QCe2xdea0Cu93C4Y3dQvaWRzAJFtmExrhj3kVMkCGzaIW0zF_JXMamxIIMFNDULKVNu6PrHcKXRF7Oyaf4LEJeU7KqGo0ksG3810SagiJLjuE6aA1Ym-IXj37YM2LhZnLmCZTTLBWnQLjrmDwWJtclBI3AHYMnG240XCeqCVvEZ1D9m071v5fnsC9ZTVUhhQso54s3cYmqOY-uFE-_AdhylGs |
link.rule.ids | 230,309,786,891,25594,76903 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dT8JADG8QjfimqFH8OhOzt4tsY2w8LEY2FhQYxEzDG7mxG2LMRmB-xL_e3jHEF31Ybtklza67ru21_RXgyuR1wxgzjdYsrtNabDIa6mP8IJplRNU4Rq0oCpx7fr39WLsfGsMCvKxqYSRO6IcER0SJGqO8Z_J_PVsfYrkyt3JxHU7xUXrjBbar5N4x2tMNVVPcpt0a9N2-oziO7fiK_2CLcIOOtkX1dgM2TXQJpav01BRVKbPfKsXbha0BUkuyPSh8PZeh5Kw6r5Vhu5cHvPE2l73FPky8eTqhEe6Ydx4RZMg0XCIts1cyE7kpESeBBJqaJrRpdlS1Q9iCiMM5MfLPPOU1Icuu0UgCr3X8mghTkKfxAVx6rcBpU3zj0Q97Ro6_Xpx-CMUkTfgRENawdBZxnakCgobjjkHPhmn1hhVWucmjY6j8Tafy3-QFlNpBrzvq3vmdE9gRbKeyqcIpFLP5Gz9DNZ2F55K_3-6Xl1U |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Frog-derived+antibacterial+peptide+Temporin-B7K11K+as+well+as+expression+method+and+application+thereof&rft.inventor=SUN+HONGHAO&rft.inventor=GAO+GAILIN&rft.inventor=SUN+XIAOHAN&rft.inventor=LYU+FUJUN&rft.inventor=ZHANG+JIAQING&rft.date=2023-09-12&rft.externalDBID=A&rft.externalDocID=CN116731120A |