Frog-derived antibacterial peptide Temporin-B7K11K as well as expression method and application thereof
The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Tem...
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Main Authors | , , , , |
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Format | Patent |
Language | Chinese English |
Published |
12.09.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Temporin-B7K11K provided by the invention is LLPIVGKLLKKLL (SEQ ID NO: 1), a codon for coding the frog-derived antibacterial peptide Temporin-B7K11K is optimized, a secretory expression vector pPIC9K and a Mut + type host bacterium Pichia pastoris GS115 are selected, a gene recombination strain for efficiently expressing the antibacterial peptide Temporin-B7K11K is constructed, fermentation conditions of recombinant pichia pastoris are optimized, and secretory expression of the antibacterial peptide Temporin-B7K11K is carried out. The antibacterial peptide is good in stability and high in expression quantity, has broad-spectrum antibacterial activity and low hemolytic activity, particularly has a relatively high killi |
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Bibliography: | Application Number: CN202310874971 |