Frog-derived antibacterial peptide Temporin-B7K11K as well as expression method and application thereof

• Main Authors: SUN HONGHAO, GAO GAILIN, SUN XIAOHAN, LYU FUJUN, ZHANG JIAQING
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of antibacterial peptides, and discloses a frog-derived antibacterial peptide Temporin-B7K11K as well as an expression method and application of the frog-derived antibacterial peptide Temporin-B7K11K. The complete sequence of the antibacterial peptide Temporin-B7K11K provided by the invention is LLPIVGKLLKKLL (SEQ ID NO: 1), a codon for coding the frog-derived antibacterial peptide Temporin-B7K11K is optimized, a secretory expression vector pPIC9K and a Mut + type host bacterium Pichia pastoris GS115 are selected, a gene recombination strain for efficiently expressing the antibacterial peptide Temporin-B7K11K is constructed, fermentation conditions of recombinant pichia pastoris are optimized, and secretory expression of the antibacterial peptide Temporin-B7K11K is carried out. The antibacterial peptide is good in stability and high in expression quantity, has broad-spectrum antibacterial activity and low hemolytic activity, particularly has a relatively high killi