Method for detecting antioxidant in water body and application

• Main Authors: BIAN RENJIE, WANG HAICHAO, WANG SHAORUI, ZHAO XIAOLI, WU FENGCHANG
• Format: Patent
• Language: Chinese; English
• Published: 08.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for determining an antioxidant in a water body and application. The method comprises the following steps: adding an AOs recovery rate indicator into a sample; then adding a mixed organic solvent composed of dichloromethane and methyl tert-butyl ether to carry out liquid-liquid extraction; concentrating the extract, replacing the solvent and concentrating; an internal standard substance Bpb-d9 is added; and determining the 28 antioxidants in the sample by using a liquid chromatograph-mass spectrometer (LC-MS/MS). The method is high in recovery rate and good in reproducibility, 28 target compounds in the water body can be enriched at the same time, LC-MS/MS is used for analyzing and quantifying AOs, and the method has the advantages of being lower in detection limit, high in detection precision and the like. 本发明公开了一种水体中抗氧化剂的测定方法与应用。本发明通过向样品中添加AOs回收率指示剂；然后加入由二氯甲烷与甲基叔丁基醚组成的混合有机溶剂进行液-液萃取；浓缩萃取液，置换溶剂并浓缩；加入内标物Bpb-d9；使用液相色谱质谱联用仪(LC-MS/MS)测定样品中的28种抗氧化剂。本发明回收率高、重现性好，能同时富集水体中28种目标化合物，使用LC