LAMP assay device and method using RNA/DNA isothermal amplification to identify pathogens

• Main Authors: ALVES PEDRO AUGUSTO, AVIRAL BRUNO SILVEIRA, ZANDONA FREITAS ANGELO USTARCHIO, RUBENS LIMA DU MONTE NETO, REZENDE MARTINS ENRIQUE, PEREIRA NEVES, HERCULES, RODRIGUES DANIELSON LAUDARES
• Format: Patent
• Language: Chinese; English
• Published: 05.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention relates to an LAMP assay device for identifying pathogens using isothermal amplification of RNA/DNA, comprising: an LAMP test chamber (1); an electronic cabinet (4) and a power supply device (7); and a top cover (2) for closing the LAMP assay chamber (1), said device comprising: a cylindrical metal dry block (8) having a hole (80) for positioning a microtube (81); a control board (14) with central processing; an electronic power board (13); at least one heating element (10) in contact with the dry block (8) and designed to heat the dry block (8) by induction; a temperature sensor (9) designed to measure the temperature of the dry block (8); a plurality of RGB LEDs (12) positioned below the dry block (8) and designed to excite each microtube positioned inside the dry block (8); and a chamber (11) positioned below the dry block (8) and designed to capture an image of each microtube (81) positioned in the dry block (8). The invention also relates to a method for identifying pathogens using