Method for immobilizing D-psicose-3-epimerase based on amination modified diatomite

• Main Authors: ZHAO ZISHEN, ZHOU LIUMING, CHEN JINGJING, XIAO ZIQUN, JIANG BO
• Format: Patent
• Language: Chinese; English
• Published: 05.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for immobilizing D-psicose-3-epimerase based on amination modified diatomite, and belongs to the technical field of enzyme engineering. The method for immobilizing the D-psicose-3-epimerase, disclosed by the invention, specifically comprises the step of immobilizing the D-psicose-3-epimerase by adopting amination modified diatomite. The fixing method is simple, efficient, economical and practical; the enzyme activity of the immobilized D-psicose-3-epimerase prepared by the method can reach 1168.98 U/g, the recovery rate is high and reaches 64.94%, the protein immobilization rate is 52.96%, and the immobilized D-psicose-3-epimerase has great potential in industrial application. 本发明公开了一种基于氨基化改性硅藻土固定D-阿洛酮糖-3-差向异构酶的方法，属于酶工程技术领域。本发明固定D-阿洛酮糖-3-差向异构酶的方法具体是采用氨基化改性的硅藻土来固定D-阿洛酮糖-3-差向异构酶；该固定方法简单高效，经济实用；并且采用该方法制备的固定化D-阿洛酮糖-3-差向异构酶酶活能够达到1168.98U/g，并且回收率高，达到64.94％，蛋白固载率为52.96％，在工业应用中具有很大的潜力。