Detection method for determining isoniazide based on dual-emission carbon quantum dot ratio fluorescent probe

• Main Authors: ZHANG JIE, MA HONGYAN, ZHANG YUECHENG, SUN LINGBO
• Format: Patent
• Language: Chinese; English
• Published: 01.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a detection method for determining isoniazide based on a dual-emission carbon quantum dot ratio fluorescent probe, and belongs to the technical field of isoniazide analysis and detection. According to the invention, the dual-emission carbon quantum dots pDECQDs are synthesized, and the ratio-type fluorescent probe is constructed, so that more accurate analysis and detection of isoniazide INH are realized. The preparation method comprises the following steps: by taking para aminobenzoic acid and o-phenylenediamine as raw materials, synthesizing the dual-emission carbon quantum dots pDECQDs with two fluorescence emission peaks at 347 nm and 555 nm by using a hydrothermal method. In a neutral medium with the pH value of 7.00, KMnO4 can effectively quench the fluorescence intensity of pDECQDs at the position of 347 nm, and the fluorescence intensity F555 at the position of 555 nm is kept basically unchanged; iNH is added into the pDECQDs-MnO4-system after the fluorescence is turned off, an