Process for screening ApoAI from human all-genome Cosmid library by PCR technique to express all genes

• Main Authors: XAOMING ZHOU, CHUNTING QI, BANGQIANG GONG
• Format: Patent
• Language: Chinese; English
• Published: 15.09.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

本发明提供了一种用PCR技术从人类全基因组Cosmid库中筛选ApoAI可表达全基因的方法。本发明首次将PCR有效扩增ApoAI的部分序列作阳性指标，应用Pool Dilution方法从Cosmid库中筛选到的人ApoAI可表达全基因作ApoAI转基因动物。