Transgenic maize event LP016-1 and detection method thereof

• Main Authors: LI SHENGBING, LI XIAOJIAO, MEI FANGMING, LU JUAN, LI SHUXIU, HAN YUYING
• Format: Patent
• Language: Chinese; English
• Published: 01.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides a nucleic acid sequence comprising one or more selected from the group consisting of sequences SEQ ID NO: 1-7 or complementary sequences thereof, said nucleic acid sequence being derived from a transgenic maize event LP016-1, a representative sample of the seed comprising said event having been preserved with preservation number CCTCC NO: P202310. The transgenic maize event LP016-1 not only has good resistance to ingestion of coleopteran pests, but also tolerates agricultural herbicides containing glufosinate-ammonium, and has the following advantages: economic loss caused by coleopteran pests is avoided; corn crops which can tolerate common commercial herbicide glufosinate-ammonium; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof. Meanwhile, the detection method provided by the invention can be used for quickly, accurately and s