Primer probe combination for detecting EB virus in nasopharynx cancer based on digital PCR

• Main Authors: REN HAOFAN, WU TIANZHUN, LU DAN, WEN BIXIA
• Format: Patent
• Language: Chinese; English
• Published: 29.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer combination for detecting EB virus and a kit thereof, the primer combination is used for detecting at least one of a first target sequence, a second target sequence and a third target sequence, the first target sequence comprises a group formed by a nucleotide sequence as shown in SEQ ID NO: 1, the second target sequence comprises a group formed by a nucleotide sequence as shown in SEQ ID NO: 5, and the third target sequence comprises a group formed by a nucleotide sequence as shown in SEQ ID NO: 6. The third target sequence comprises a group formed by a nucleotide sequence as shown in SEQ ID NO: 9. The primer provided by the invention can obviously improve the sensitivity of clinical detection of the EB virus, and particularly has relatively high accuracy for detection of an early sample. 一种用于检测EB病毒的引物组合及其试剂盒，所述引物组合用于检测第一目标序列、第二目标序列、第三目标序列中的至少一种，所述第一目标序列包含SEQ ID NO:1所示核苷酸序列所构成的组，所述第二目标序列包含SEQ ID NO:5所示核苷酸序列所构成的组，所述第三目标序列包含SEQ ID NO:9所示核苷酸序列所构成的组。发明提供的引物可以明显提高临床检测EB病毒的灵敏度，特别是针