Method for primary culture of mouse dorsal root ganglion satellite glial cells

• Main Authors: LUO QINGTIAN, LI NAN, ZHU QING, CHENG CHUNSHENG, WANG SASHUANG, JIANG CHANGYU, LIAO XIANG
• Format: Patent
• Language: Chinese; English
• Published: 29.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for primary culture of mouse dorsal root ganglion satellite glial cells, which comprises the following steps: preparing a 10X digestive enzyme storage solution, dissolving 10mg/mL collagenase, 4mg/mL pancreatin and 1mg/mL DNA enzyme I in a high-glucose DMEM basal culture medium, subpackaging into 100 [mu] L per tube, and preserving at-20 DEG C, and preparing a complete culture medium, a formula of the culture medium comprises a high-glucose DMEM basal culture medium, 10% fetal calf serum, 100U/ml penicillin and 0.1 mg/mL, and the culture medium is preserved at 4 DEG C. The purified DRG satellite glial cells can be obtained only in 7 days, the required time is short, an adult mouse DRG satellite glial cell separation culture system is established by adopting a DRG tissue primary culture method, a high-purity DRG satellite glial cell purification culture system can be obtained, and the cultured and purified DRG satellite glial cells are long in in-vitro survival time, high in pu