MiR-3074-3p inhibitor and application thereof

• Main Authors: SONG WENJING, SHEN JINGJIE, LUO XINGHONG, MA DANDAN, JIANG TAO, MIAO SHENGHONG, TAN SHENGLONG
• Format: Patent
• Language: Chinese; English
• Published: 29.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a miR-3074-3p inhibitor and an application of the miR-3074-3p inhibitor. According to the scheme, the regulating effect of the miR-3074-3p in the dental pulp stem cell dental differentiation process is found for the first time, and the dental pulp stem cell dental differentiation can be promoted by preparing the inhibitor AntamiR-3074-3p of the miR-3074-3p; according to the present invention, the AntagomiR-3074-3p/PEI-AuNPs compound is constructed on the basis, has good biocompatibility, can effectively promote the dental differentiation of human dental pulp stem cells in vitro, can be used as the pulp capping agent by further constructing the AntagomiR-3074-3p/PEI-AuNPs/GELMA compound, can significantly promote the dentin formation effect in the rat body, and further can be used for dentin repair and regenerative treatment. 本发明公开了一种miR-3074-3p抑制剂及其应用。本发明方案首次发现了miR-3074-3p在牙髓干细胞成牙分化过程中的调节作用，通过制备miR-3074-3p的抑制剂AntagomiR-3074-3p可促进牙髓干细胞成牙分化；在此基础上构建了AntagomiR-3074-3p/PEI-AuNPs复合物，具有良好的生物相