Loquat cold-resistant gene EjGLK1 as well as encoded protein and application thereof

• Main Authors: SEO HUN, LIU MINGXIU, YANG HAO, LIANG GUOLU, GUO QIGAO, JING DANLONG, SUO XIAODONG
• Format: Patent
• Language: Chinese; English
• Published: 25.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the field of plant molecular biology, in particular to a loquat leaf cold resistance related gene EjGLK1, a protein coded by the same and application of the EjGLK1. The full length of the cDNA sequence of the gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoded protein of the gene is as shown in SEQ ID No.2. According to the EjGLK1 gene disclosed by the invention, the cold resistance of plants is improved by influencing the synthesis of chloroplast related genes and the expression of related genes in a low-temperature stress pathway ICE-CBF-COR. A plant expression vector pFGC5941-35S:: EjGLK1 containing a target gene is transferred into wild arabidopsis thaliana through agrobacterium tumefaciens mediation by using a flower immersion method. The result shows that the overexpression of EjGKL1 enhances the cold resistance of arabidopsis thaliana. Compared with an arabidopsis thaliana GLK gene-deficient material, the transgenic arabidopsis thaliana plant material ob