Molecular marker of brassica napus low-temperature male sterility site as well as primer pair and application of molecular marker

• Main Authors: YANG MAO, JIANG XIAOMEI, CHEN JUN, CAO LIFANG, GUAN RONGZHAN
• Format: Patent
• Language: Chinese; English
• Published: 22.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a molecular marker of a brassica napus low-temperature male sterility site as well as a primer pair and application of the molecular marker. According to the invention, a low-temperature male sterility site Bnlst1 of the rape is positioned on an A07 chromosome through a map-based cloning detection method and is positioned between BnInDel06 and BnInDel24, and the site is co-separated from BnInDel12, BnInDel18, BnInDel20 and BnInDel23. The low-temperature male sterility character of the brassica napus is co-separated from the four molecular markers, and the genotype and phenotype of a single plant are consistent, so that the four molecular markers have a huge application prospect in the assisted selection breeding of the low-temperature male sterility line of the brassica napus, and can be used for effectively and quickly screening low-temperature male sterility materials and distinguishing fertile materials in the seedling stage of the brassica napus; the selection efficiency is improve