Bacillus subtilis protease and preparation method thereof

• Main Authors: ZHANG SHUWEN, WU JUNRUI, YANG HUI, LOU MENGXUE, ZHANG TAOWEI, JI SHUAIQI, PAN GUOYANG, LYU JIAPING, HE KAIRU, WU RINA
• Format: Patent
• Language: Chinese; English
• Published: 22.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MAKING THEREOF; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MILK OR CHEESE SUBSTITUTES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of functional microorganism application, in particular to bacillus subtilis protease and a preparation method thereof. The protease is generated by bacillus subtilis SNBS-3, has good stability in a neutral or slightly alkaline environment, has a very strong inhibition capability on listeria monocytogenes, has a remarkable in-vitro thrombolysis effect and a remarkable dissolving effect on milk protein, and has a wide application prospect. 本发明涉及功能微生物应用技术领域，具体涉及一种枯草芽孢杆菌蛋白酶及其制备方法。所述蛋白酶由枯草芽孢杆菌(Bacillus subtilis)SNBS-3产生，在中性或偏碱性的环境下有良好的稳定性，对单增李斯特菌具有很强的抑制能力，体外溶栓效果和对乳蛋白的溶解效果显著，应用前景广阔。