Hybridoma cell and application thereof in preparation of monoclonal antibody secreting mouse anti-human procalcitonin

• Main Authors: LIU XUEQIN, WU MENG, LANG QIAOLI, JIANG YU, GE LIANGPENG, LUO LIN, XIAO PUYING
• Format: Patent
• Language: Chinese; English
• Published: 22.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a hybridoma cell strain. The mouse anti-human procalcitonin monoclonal antibody with good specificity and strong affinity can be obtained. The invention also provides a preparation method of the mouse anti-human procalcitonin monoclonal antibody, the mouse anti-human procalcitonin monoclonal antibody obtained by the hybridoma cell disclosed by the invention is used for producing the antibody through a hybridoma technology and a recombination technology, the yield is high, and the purity of the antibody product is high and reaches 95% or above. The invention also provides a kit containing the anti-human procalcitonin monoclonal antibody, the kit for detecting human procalcitonin has the advantages of high specificity and sensitivity, and can be used for auxiliary diagnosis of inflammatory diseases and evaluation of treatment prognosis. 本发明提供了一种杂交瘤细胞株，可获得特异性好，亲和力强的鼠抗人降钙素原单克隆抗体。本发明还提供了一种鼠抗人降钙素原单克隆抗体的制备方法，采用本发明杂交瘤细胞获得的鼠抗人降钙素原单克隆抗体，通过杂交瘤技术和重组技术生产抗体，产量高，抗体产物纯度高，达95%以上。本发明还提供了包含所述抗人降钙素原单克隆抗体的试