Topological protein based on isohydrocarbon skeleton, preparation method and application

The invention discloses a topological protein based on an isohydrocarbon skeleton, and a preparation method and application thereof, and belongs to the technical field of life science. The isohydrocarbon skeleton is composed of a plurality of skeleton units, the skeleton units are BTA or ATB, the BT...

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Bibliographic Details
Main Authors TIAN XIBAO, ZHANG WENBIN
Format Patent
LanguageChinese
English
Published 22.08.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a topological protein based on an isohydrocarbon skeleton, and a preparation method and application thereof, and belongs to the technical field of life science. The isohydrocarbon skeleton is composed of a plurality of skeleton units, the skeleton units are BTA or ATB, the BTA sequentially comprises SpyCatcher, anti-TRAP and SpyTag from the N end to the C end, the ATB is reversed in sequence, and both the BTA and the ATB are of closed-loop structures and free from the N end and the C end. The preparation method comprises the following steps: firstly, synthesizing a Soxhdrocarbon skeleton, and then developing the Soxhdrocarbon skeleton as a template into a trivalent display system of a plurality of active proteins, so that the preparation of the bispecific hexavalent antibody is realized, and the preparation method is a novel protein coupling strategy. On the basis of reserving the protein structure and activity, the affinity of an affinity body is improved through a multivalent effect
Bibliography:Application Number: CN202310881917