Methods for cryopreservation of engineered Tregs

• Main Author: MCGILL, IAIN
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method of preserving CD62L expression in a regulatory T cell (Treg) population that has been cryopreserved, comprising introducing a polynucleotide encoding a FOXP3 polypeptide into the Treg population prior to cryopreservation. The present invention also relates to a method of preserving CD62L in a cryopreserved Treg population, comprising introducing a polynucleotide encoding a FOXP3 polypeptide into the Treg population and cryopreserving the Treg population. Furthermore, the present invention relates to the use of an exogenous polynucleotide encoding FOXP3 for preserving CD62L expression in a cryopreserved Treg population, and to a cryopreserved engineered Treg population, a pharmaceutical composition comprising the cryopreserved engineered Treg, and its therapeutic use. 本发明涉及将CD62L表达保存在已经冷冻保存的调节性T细胞(Treg)群中的方法，包括在冷冻保存之前将编码FOXP3多肽的多核苷酸引入Treg群中。本发明还涉及将CD62L保存在冷冻保存后的Treg群中的方法，包括将编码FOXP3多肽的多核苷酸引入Treg群中并且冷冻保存所述Treg群。此外，本发明涉及编码FOXP3的外源多核苷酸在用于将CD62L表达保存在冷冻保存后的Treg群中的用途，并且涉及冷冻保存