Non-nucleic acid extraction type qPCR (quantitative polymerase chain reaction) detection method for active ruminococcus in fecal sample

• Main Authors: GU DAYONG, ZHAO WENWU, XU TING, ZHUANG TIANCHI, YANG PEI, ZHANG ZHAN, JI MINGHUI, ZHAO YANJIE, LI NING, LIU YUN, CAO WEI, SUN TAO, XU QIN, GU YIBO
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a non-nucleic acid extraction type qPCR (quantitative polymerase chain reaction) detection method for active ruminococcus in a fecal sample, which is free of a nucleic acid extraction step, optimizes an amplification enzyme, an active ruminococcus fluorescent quantitative PCR reaction system and reaction conditions, can detect active ruminococcus, and is used for rapidly and accurately detecting active ruminococcus in an intestinal sample. 本发明公开了一种粪便样本中活泼瘤胃球菌的非核酸提取式qPCR检测方法，免核酸提取步骤，并优化了包括扩增酶、活泼瘤胃球菌荧光定量PCR反应体系和反应条件，可以检测出活泼瘤胃球菌，用于快速、准确地检测肠样本中的活泼瘤胃球菌。