Optofluidic array for free radical protein footprint

• Main Authors: EGGEN RICHARD W, HOLMAN DAVID A, WEINBERG SCOTT R, PESHOFF JOSEPH JOHN, XIE ERNEST E, BUI THANH HUY
• Format: Patent
• Language: Chinese; English
• Published: 15.08.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Systems and methods for in vivo and in vitro free radical protein footprint using an optofluidic array are presented. These teachings can be used, for example, for studying three-dimensional protein structures or biodynamics. Free radical dosimetry, including optional internal standards, is used. Real-time feedback based on the internal standard provides comparability between different experiments, as well as in vivo and in vitro analysis results representing actual biological conditions. 提出了使用光流控阵列的体内和体外自由基蛋白质足迹法的系统和方法。这些教导可以用于例如研究三维蛋白质结构或生物动力学。使用包括可选内标在内的自由基剂量测定法。基于内标的实时反馈提供了不同实验之间的可比性、以及表示实际生物条件的体内和体外分析结果。