Method for screening cattle cold climate adaptation gene DNAJC18 and functional molecular marker and application thereof

• Main Authors: YANG CHUNHONG, WEI XIAOCHAO, LIU WENHAO, HUANG JINMING, HUANG NING, WANG JINPENG, ZHAO LIHONG, ZHANG YARAN, GAO YAPING, XIAO YAO, JU ZHIHUA
• Format: Patent
• Language: Chinese; English
• Published: 15.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of animal genetic breeding, and particularly relates to a method for screening a cattle cold climate adaptive gene DNAJC18 and a functional molecular marker and application of the cattle cold climate adaptive gene DNAJC18. According to the invention, cattle varieties distributed in north and south are selected, a cattle high-density SNP chip is used for genetic typing, two genomic selection signal analysis methods of FLK and hapFLK are integrated, genomic selection signals between the south and north cattle and in the cattle varieties are compared and analyzed, and strongly selected areas and positive selection genes are identified. Screening out a gene adapting to a cold environment and a molecular marker thereof; potential functional markers are further mined by utilizing re-sequencing data of the cattle, and a corresponding detection technology is established. The invention provides a scientific basis and a simple and feasible detection technology for molecular