Steroid C1, 2 dehydrogenation reaction engineering strain with excellent transport and reaction activity

• Main Authors: WANG MIN, AI LU, ZHU WENCHENG, LUO JIANMEI, SHEN YANBING, SONG ZHAOYU, JIA HONGCHEN
• Format: Patent
• Language: Chinese; English
• Published: 15.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a steroid C1, 2 dehydrogenation reaction engineering strain with excellent transport and reaction activity, and the transport capacity and stress tolerance of the strain are improved by overexpressing a transport protein or co-expressing the transport protein and a heat shock protein in arthrobacter simplex. The obtained engineering strain still keeps good catalytic activity in a conversion system of a high-concentration substrate and an organic solvent, and the C1, 2 dehydrogenation reaction yield is increased by 1.42%-51.74% compared with that of a control strain. The engineering strain with strong transport capacity and stress tolerance improves the concentration of a substrate and an organic solvent in a conversion system, further improves the production amount of a product, and has important guiding significance for improving the conversion efficiency of a high-concentration substrate of a steroid hydrophobic compound. 本发明涉及一种具有优良转运和反应活性的甾体C1,2脱氢反应工程菌株，通过在简单节杆菌中过表达转运蛋白、或者共表达转运蛋白和