Modified recombinant monoclonal IgM antibody of HIV-1 (human immunodeficiency virus-1) neutralizing antibody and application of modified recombinant monoclonal IgM antibody

• Main Authors: WAN XINRUI, LI YA, DUAN YONG, ZHANG RUIXIAN
• Format: Patent
• Language: Chinese; English
• Published: 15.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a modified recombinant monoclonal IgM antibody of an HIV-1 (human immunodeficiency virus-1) neutralizing antibody and application of the modified recombinant monoclonal IgM antibody. The modified recombinant monoclonal IgM antibody of the HIV-1 neutralizing antibody comprises (a) a heavy chain constant region, and the amino acid sequence of the IgM heavy chain constant region is as shown in SEQ ID NO: 1; (b) a light chain constant region, (c) a heavy chain variable region, and (d) a light chain variable region. According to the neutralizing antibody modified by the IgM, not only is the affinity of the antibody improved, but also the neutralizing activity of the antibody is indirectly improved, and more strategies are provided for prevention of HIV-1 and vaccine development. 本发明公开了一种HIV-1中和抗体的改造重组单克隆IgM抗体及其应用。本发明的一种HIV-1中和抗体的改造重组单克隆IgM抗体，包括(a)重链恒定区，所述IgM重链恒定区的氨基酸序列是SEQ ID NO:1所示的氨基酸序列；(b)轻链恒定区，(c)重链可变区，(d)轻链可变区。本发明IgM改造后的中和抗体不仅提高了抗体的亲和力，同时间接提高了抗体的中和活性，为HIV-1的预防和疫苗开发提供更多的策略。