Method for noninvasively infecting trichogramma with Wolbachia by using artificial hosts

• Main Authors: YAN ZHICHAO, HONG XIAOYUE, LI YUANXI
• Format: Patent
• Language: Chinese; English
• Published: 11.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW BREEDS OF ANIMALS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TRAPPING

The invention belongs to the technical field of biology, and particularly relates to a method for noninvasively infecting trichogramma with Wolbachia by using an artificial host, which comprises the following steps: firstly preparing an artificial host egg card, then inoculating a biological material infected with Wolbachia into the artificial host egg card, and finally inoculating trichogramma parasites not infected with Wolbachia. Compared with microinjection, the method is more convenient and efficient; meanwhile, an open system capable of being operated by an artificial host provides possibility for artificial infection of Wolbachia from other insects except for egg parasitic wasps by trichogramma. 本发明属于生物技术领域，具体涉及一种利用人工寄主向赤眼蜂无创侵染Wolbachia的方法，首先制备人工寄主卵卡，接着将感染Wolbachia的生物材料接入人工寄主卵卡，最后接入未感染Wolbachia的赤眼蜂寄生，即可。本方法相较于显微注射，更便捷高效；同时，人工寄主可操作的开放体系，为赤眼蜂人工侵染卵寄生蜂外其他昆虫来源的Wolbachia提供了可能。