Method for separating molecules and/or molecular complexes

• Main Authors: VIEUX CHRISTOPHE, CAYRON HELENE, BARBIE MICKAEL, ESTERLAD DAVID, LAROCHE ALEXANDRE, ZELF ANDREAS
• Format: Patent
• Language: Chinese; English
• Published: 08.08.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for the in vitro separation of molecules and/or molecular complexes having a radius of gyration of less than or equal to 2 [mu] m from a complex fluid, said method comprising the following steps: a) contacting the complex fluid with a structured capture array having topographic features in the form of a plurality of planar surfaces located between cavities, wherein the structured capture array is surrounded by wet air; b) covering the deposited complex fluid with a covering device such that the complex fluid is surrounded by a meniscus comprising a posterior meniscus and an anterior meniscus wherein a surface tension of the complex fluid located between the covering device and the structured capture array defines at least the anterior meniscus and the posterior meniscus; c) dragging the covering device or the structured capture array in a direction at a speed of at most 2 mm.s'-1 to displace the complex fluid wherein the anterior meniscus and the posterior meniscus are displa