Carbofuran aptamer electrochemical sensor based on sensibilization effect of three-dimensional hierarchical porous carbon material and application method of carbofuran aptamer electrochemical sensor

• Main Authors: LUO LIHONG, JIANG CUIWEN, LIANG ZHONGDAN, LAO SHUIBING, YA YU
• Format: Patent
• Language: Chinese; English
• Published: 08.08.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a carbofuran aptamer electrochemical sensor based on the sensibilization effect of a three-dimensional hierarchical porous carbon material and an application method of the carbofuran aptamer electrochemical sensor. The preparation method of the carbofuran aptamer electrochemical sensor comprises the following steps: (1) preparing the three-dimensional hierarchical porous carbon material; (2) preparation of AuNPs (at) HPC; (3) preparing an AuNPs (at) HPC/Chit dispersion liquid; (4) preparing a Tris-HC buffer solution; (5) preparation of 1 mMol/L < 6 >-sulfydryl-1-hexanol; (6) preparing a 5.0 mMol/L [Fe (CN) 6] < 3-/4-/0.1 Mol/L KCl solution; (7) preparing a carbofuran nucleic acid aptamer solution; and (8) preparing the carbofuran aptamer electrochemical sensor. The invention also provides an application method of the carbofuran aptamer electrochemical sensor. The invention establishes an ultra-sensitive and high-selectivity carbofuran aptamer electrochemical sensor, the electrochemical