Gene segment for enhancing lodging resistance of pepper, and encoded protein, detection kit, rapid detection method and application thereof

• Main Authors: FU CANFANG, LI QING, SUO HUAN, DAI XIONGZE, OU LIJUN, YANG BOZHI, LIU ZHOUBIN, ZOU XIAOXUE
• Format: Patent
• Language: Chinese; English
• Published: 08.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a capsicum lodging-resistant gene, a specific sequence thereof and an application of an encoded protein of the capsicum lodging-resistant gene in enhancing the lodging-resistant performance of capsicum, chemical mutagenesis is carried out on wild capsicum 6421 to discover a stalk creeping mutant sp1, a gene with remarkable differential expression is obtained after analysis, the gene is named as CaMYB61, and the specific sequence of the capsicum lodging-resistant gene is named as CaMYB61. The invention also provides a nucleotide sequence and an amino acid sequence of the gene. The CaMYB61 gene provided by the invention provides reference for breeding of a new lodging-resistant pepper variety, and has a positive effect on lodging-resistant breeding of the pepper. 本发明提供一种辣椒抗倒伏基因、其特异性序列及其编码蛋白在增强辣椒抗倒伏性能中的应用，对野生型辣椒6421进行化学诱变发现茎秆匍匐突变体sp1，分析后得到一个显著差异表达的基因，将该基因命名为CaMYB61，同时提供了该基因的核苷酸序列和氨基酸序列。本发明中的CaMYB61基因为辣椒抗倒伏新品种的选育提供了参考，对辣椒抗倒伏育种具有积极作用。