Method for preparing chiral lactam compound by using aminotransferase mutant

• Main Authors: WANG NAN, MENG XIAO, WEN XIANGHUI, ZHU LU, DAI DANMEI, ZHU JINGYANG, LIU CHAO
• Format: Patent
• Language: Chinese; English
• Published: 08.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for preparing a chiral lactam compound by using an aminotransferase mutant. Specifically, the method comprises the following steps: in the presence of an aminotransferase mutant, carrying out amination reaction on a compound as shown in a formula I or a salt thereof and an amino donor, and carrying out amination reaction in a reaction solvent to obtain a compound II, the amino acid sequence of the aminotransferase is as shown in SEQ ID NO: 1; the mutant has the difference of amino acid residues selected from one or more sites on an amino acid sequence as shown in SEQ ID NO: 1. The invention also discloses an aminotransferase mutant and a reaction process for synthesizing the beta-(S) chiral lactam compound developed based on the aminotransferase mutant, the reaction can be carried out at a relatively high temperature (55 DEG C), the enantiomer excess value (e.e.) is greater than or equal to 90%, and the yield is greater than 50%. 本发明公开了一种使用氨基转移酶突变体制备手性内酰胺类化合物的方法。具体地，所述方法包括以下步骤