Method for improving production of L-lactic acid by saccharomyces cerevisiae based on regulation and control of ethanol metabolic flux

• Main Authors: PLUGOONG KOK SENG, LIU TIANTIAN, CHEN JIAN, LIU YANFENG, LI JIANGHUA, LIU LONG, LYU XUEQIN
• Format: Patent
• Language: Chinese; English
• Published: 08.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for improving production of L-lactic acid by saccharomyces cerevisiae based on regulation and control of ethanol metabolic flux, and belongs to the technical field of microorganisms. The acid-resistant saccharomyces cerevisiae TJG16 is used as a production strain and has the acid resistance of saccharomyces cerevisiae in the organic acid fermentation process, and the yield of L-lactic acid is greatly increased. Saccharomyces cerevisiae TJG16 is improved, an ethanol dehydrogenase gene adhA sourced from bacillus subtilis is introduced to promote conversion of ethanol into acetaldehyde, and a lactic aldolase gene BAL sourced from Brucella is introduced to promote synthesis of lactic acid from acetaldehyde. According to the method, L-LA is used as a raw material, an acetaldehyde dehydrogenase gene ALD6 is knocked out to prevent acetaldehyde from synthesizing acetic acid, a transcriptional regulatory factor encoding gene GAL80 for regulating galactose is knocked out, lactic dehydro