Endoclita serpta vacuole ATPase A subunit (PeV-ATPase A) gene, dsRNA thereof, synthetic method and insect resistance application of endoclita serpta vacuole ATPase A subunit (PeV-ATPase A) gene

• Main Authors: ZHAN YAGUANG, JING TIANZHONG, QI FENGHUI, ZENG FANSUO, WANG WENXUAN
• Format: Patent
• Language: Chinese; English
• Published: 04.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an endoclyta lophila vacuole ATPase A subunit (PeV-ATPase A) gene, dsRNA thereof, a synthetic method and insect resistance application thereof.The endoclyta lophila vacuole ATPase A subunit (PeV-ATPase A) gene is characterized in that a specific primer pair SEQ ID NO: 4 and SEQ ID NO: 5 of an open reading frame of the PeV-ATPase A gene is designed and synthesized through transcriptome analysis, a PeV-ATPase A gene sequence is obtained through gene cloning, the nucleotide sequence is SEQ ID NO: 1, and the amino acid sequence is SEQ ID NO: 2; then selecting a gene segment with a sequence of SEQ ID NO: 3 as an RNAi target sequence, designing and synthesizing a primer pair SEQ ID NO.6 and SEQ ID NO.7 with enzyme cutting sites, carrying out PCR amplification, constructing a prokaryotic expression vector, and inducing dsRNA expression. The expression of the PeV-ATPase A gene is interfered by feeding dsRNA (double-stranded ribonucleic acid) to the endoclyta lolyta larvae, so that the endoclyt