Cell wall defect yeast engineering bacterium as well as construction method and application thereof

• Main Authors: LIANG SHIYU, LYU LITING, ZHANG YUE, ZHAO ZONGBAO
• Format: Patent
• Language: Chinese; English
• Published: 04.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses cell wall defect yeast engineering bacteria as well as a construction method and application thereof. The cell wall defect yeast engineering bacteria are obtained by introducing a DNA (Deoxyribose Nucleic Acid) fragment containing a cell wall hydrolase gene into an original strain; the cell wall hydrolase can secrete and express cell wall hydrolase and continuously crack the cell wall to cause cell wall damage of engineering bacteria, so that intracellular grease extraction is effectively promoted, the problem that cell disruption and intracellular grease extraction are hindered due to compact cell wall structure of oleaginous yeast is fundamentally solved, and the method has wide industrial application prospects. 本申请公开了一种细胞壁缺陷酵母工程菌及其构建方法和应用，所述细胞壁缺陷酵母工程菌通过在出发菌株中导入含有细胞壁水解酶基因的DNA片段得到；可以分泌表达细胞壁水解酶，持续裂解自身细胞壁导致工程菌细胞壁损伤，从而有效促进胞内油脂提取，从根本上解决产油酵母细胞壁结构致密阻碍细胞破碎和胞内油脂提取的问题，具有广泛的工业应用前景。