Adipose mesenchymal exosome and preparation method thereof

• Main Authors: HE LASER, JI ZIYUN, LI JIE, LIU CHANG, HE YANLING, CHEN ZHENGLIN
• Format: Patent
• Language: Chinese; English
• Published: 01.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an adipose mesenchymal exosome and a preparation method thereof, and belongs to the technical field of application of lung injury, a 25ml transfer pipette is used for sucking lower-layer liquid in an adipose sample in a collection bottle which is layered after standing and discarding the lower-layer liquid, then a 25ml transfer pipette is used for sucking adipose tissues, 20-25ml of adipose tissues with the same volume are added into each centrifugal tube, a cover is covered, and the adipose mesenchymal exosome is obtained. And (3) carrying out centrifugal treatment on the adipose tissue in the step (3), dividing the adipose tissue into an upper layer, a middle layer and a lower layer after centrifugation, sucking and discarding grease on the upper layer and cleaning fluid on the lower layer, sucking the adipose tissue into a clean centrifugal tube by using a 25ml pipette, adding the same amount of adipose tissue into each tube, keeping away from white fascia tissue, and uniformly shak