Application of knockout sigW gene in improving production of Protein A protein by bacillus licheniformis

• Main Authors: XIE XIAOMEI, XU HAIXIA, WANG DONG, ZHAN YANGYANG, CHEN SHOUWEN, CAI DONGBO, LI TAO
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of gene engineering, and discloses application of knockout sigW gene in improvement of Protein A protein production of bacillus licheniformis. According to the invention, a sigW gene in bacillus licheniformis BL10 is knocked out to construct a recombinant strain BL10 [delta] sigW, and an expression vector derived from a staphylococcus aureus recombinant protein Protein A is introduced on the basis of the recombinant strain BL10 [delta] sigW. Compared with a control strain, the deletion of the sigW gene can specifically improve the expression quantity of the recombinant Protein A. The recombinant Protein A disclosed by the invention can be widely applied to an immunoglobulin affinity chromatography technology. The bacillus is used for expressing the recombinant Protein A for the first time, no bacterial endotoxin is generated, the sigW is knocked out, the yield of the Protein A is greatly increased, the production cost is remarkably reduced, and a new thought and a