Methods of identifying markers of graft rejection

• Main Authors: KRISHNA KUMAR SURESH, LEE MEELAN, PALSGAARD PETER, MINDRINOS MARK N
• Format: Patent
• Language: Chinese; English
• Published: 25.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to polynucleotide probes, each comprising two strands that are perfectly complementary. In some embodiments, each of the strands comprises, in the 5'to 3 'direction, a) a first target hybridization sequence, b) a first digital tag sequence, c) a first Halo barcode sequence, d) a first Halo amplification primer sequence, e) a reverse second Halo amplification primer sequence, f) a reverse second Halo barcode sequence, g) a reverse second digital tag sequence, and h) a reverse second target hybridization sequence. The invention also relates to methods of using these novel probes to determine the level of a minor population of DNA in a mixture of DNA from two different sources. 本发明涉及多核苷酸探针，每个多核苷酸探针包含两条完美互补的链。在一些实施方案中，该链中的每一个以5'至3'方向包含，a)第一靶杂交序列，b)第一数字标签序列，c)第一Halo条形码序列，d)第一Halo扩增引物序列，e)反向第二Halo扩增引物序列，f)反向第二Halo条形码序列，g)反向第二数字标签序列，和h)反向第二靶杂交序列。本发明还涉及使用这些新的探针来确定来自两个不同来源的DNA的混合物中DNA次要群体的水平的方法。