Method for detecting glucoraphanin in broccoli seeds by using high performance liquid chromatography

• Main Authors: LI HUI, SHEN JUNZI, WANG YA, FENG BAOJUN, LIU FANG, CHU HAOXIA
• Format: Patent
• Language: Chinese; English
• Published: 25.07.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting glucoraphanin in broccoli seeds by using a high performance liquid chromatography technology, and belongs to the technical field of chemical analysis and detection.The method comprises the following steps that S1, a reference substance is prepared, specifically, a proper amount of glucoraphanin standard substance is taken and dissolved with methanol, and a reference substance solution with a certain concentration is prepared; s2, pretreatment of a test sample: crushing broccoli seeds, immediately adding boiling water for extraction, and fixing the volume to a certain volume; s3, chromatographic detection: a mobile phase is methanol, 0.1% phosphoric acid is used for gradient elution, 5% methanol is kept in 0-8 min, 5%-95% methanol is kept in 8.1-13 min, and 95%-5% methanol is kept in 13.1-22 min; the sample size is 10 [mu] L; the column temperature is 30 DEG C; the flow rate is 1mL/min; and quantifying by using a standard curve. Under the detection condition of th