Method for inducing plasma stem cells to secrete cytokines

• Main Authors: FANDRIDGE FRED, HU JIANJIE, XU ZI'EN
• Format: Patent
• Language: Chinese; English
• Published: 21.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for inducing plasma stem cells to secrete cytokines. According to the requirements of cell culture and induced culture, a complete culture medium and an induced culture medium are provided, meglumine, dextran and dipotassium glycyrrhizinate are added into the complete culture medium, all the components have a synergistic effect, induced culture is preliminarily completed in the stem cell culture process, secretion of cell factors is promoted, and the stem cell culture efficiency is improved. The content of cell factors in the cell culture fluid is increased; the induction culture medium takes zeatin, transferrin, sodium alginate, glutamine and glucose as main components, can effectively promote secretion of cell factors of stem cells and improve the content of the cell factors in a culture solution, the whole process is beneficial to collection and application of the cell factors, operation is easy, cost is saved, and the method is suitable for industrial production. And comm