Novel light-cleavable mass tags for tissue multiplexed mass spectrometry imaging using biomolecular probes

• Main Authors: YAGNIK GARY, ROTHSCHILD KEVIN J, M. J. RING
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Immunohistochemical (INC) and in situ hybridization (ISH) methods for, for example, targeted detection and localization of biomolecules (e.g., proteins and miRNAs) in tissues or cells, for research applications and for clinical applications such as pathologies (e.g., biomarker analysis of resected tumors or tumor biopsy). The methods include mass spectrometry imaging (MSI) as a pattern for detecting and localizing biomolecules in tissue or cells. The methods employ light-cleavable quality tag reagents linked to probes such as antibodies and nucleic acids and used to achieve multiplexed immunohistochemistry and in situ hybridization with MSI as the detection/readout mode. The method also includes a multi-omics MSI procedure in which the MSI of the light-cleavable quality tag probe is combined with other MSI patterns, such as a direct label-free MSI of endogenous biomolecules from a biological sample (e.g., tissue). 免疫组织化学(INC)和原位杂交(ISH)方法，其用于例如组织或细胞中生物分子(例如，蛋白和miRNA)的靶向检测和定位，用于研究应用和用于诸如病理学家的临床应用(例如，切除的肿瘤或肿瘤活组织