Construction method and detection method of characteristic chromatogram of caulis clematidis armandii standard decoction

• Main Authors: LIU XIAOXIA, LV WEISHENG, DENG CONGYOU, DUAN ZHIWEN, CHEN XIANGDONG, LU XIAOYING, HE MINYOU, LEE JIN-WOO, SUN DONGMEI, YANG JIE
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a construction method of a specific chromatogram of a caulis clematidis armandii standard decoction, which comprises the following steps: taking freeze-dried powder of the caulis clematidis armandii standard decoction to prepare a test solution; performing high performance liquid chromatography analysis on the test solution, and constructing a characteristic chromatogram of the caulis clematidis armandii standard decoction; wherein the conditions of the high performance liquid chromatography are as follows: a mobile phase A is acetonitrile, and a mobile phase B is water; gradient elution is adopted; the gradient elution procedure is as follows: the volume percentage of the mobile phase A is 3% in 0-5 min; the volume percentage of the mobile phase A is changed from 3% to 18% in 5-20 min; the volume percentage of the mobile phase A is changed from 18% to 22% in 20-30 min; and the volume percentage of the mobile phase A is changed from 22% to 35% in 30-60 min. 本发明提供了一种川木通标准汤剂的特征图谱的构建方法，包括如