Method for extracting ergoselenine and selenoprotein from spirulina

The method comprises the following steps: culturing an FACHB-Se1500 algal strain under the nitrogen-deficient induction condition to obtain a product, purifying the product to obtain the selenoprotein containing the ergoselenine, culturing the FACHB-Se1500 algal strain in a Zarrouk + Se culture medi...

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Bibliographic Details
Main Authors WU SHENGWU, LONG HUAN, HUANG KAIYAO, ZHANG BAOLONG, LI CHAOYANG
Format Patent
LanguageChinese
English
Published 18.07.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The method comprises the following steps: culturing an FACHB-Se1500 algal strain under the nitrogen-deficient induction condition to obtain a product, purifying the product to obtain the selenoprotein containing the ergoselenine, culturing the FACHB-Se1500 algal strain in a Zarrouk + Se culture medium for 10 days under the conditions that the temperature is 25-30 DEG C, the pH value is 9.5 and the illumination intensity is 40 [mu] E.M <-2 >. S <-1 >, and after a plateau phase is reached, culturing the FACHB-Se1500 algal strain in the Zarrouk + Se culture medium to obtain the selenoprotein containing the ergoselenine. The preparation method comprises the following steps: respectively transferring into a Zarrouk-N culture medium, a Zarrouk-Fe culture medium and a Zarrouk-Mg culture medium, carrying out induced culture for 2 days, collecting algae powder, and purifying to obtain selenoprotein containing ergoselenine. According to the invention, a spirulina mutant strain with high selenium resistance is obtained
Bibliography:Application Number: CN202211724747