High-activity rumen cellulase and gene thereof

• Main Authors: ZHOU ZIJIAN, ZHENG JIEYI, YANG SHULI, ZHU YAXIN, ZHANG GUANGRONG, GOU XIAO
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention relates to a high-activity rumen cellulase and a gene thereof, and belongs to the field of gene engineering, the amino acid sequence of the high-activity cellulase Umcel-2 is shown as SEQ ID NO.1, and the nucleotide sequence of the coding gene of the high-activity cellulase is shown as SEQ ID NO.2. The Umcel-2 gene is obtained from the rumen of the large-forehead cattle, the activity of cellulase can be expressed, and the Umcel-2 gene has a degradation effect on bamboo powder, straw and alfalfa, so that the Umcel-2 gene can be used for deeper understanding of efficient degradation of cellulase coded by the cellulase gene in the rumen of the large-forehead cattle on crude fiber feed such as bamboos and the like; and certain theoretical basis and practical significance are provided for developing a plurality of high-efficiency cellulase genes into novel cellulose compound enzyme resources by means of molecular biology and the like. 本发明涉及一种高活性的瘤胃纤维素酶及其基因，属于基因工程领域，该高活性纤维素酶Umcel-2的氨基酸序列如SEQ ID NO.1所示