Integrated method for sampling-capturing microorganisms in aerosol by using integrated sampling enrichment equipment and immunomagnetic bead incubation technology

• Main Authors: QU XIAOYUN, HU YAPING, PAN JINMING, FU YINGCHUN, YING YIBIN
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an integrated method for sampling-capturing microorganisms in aerosol by using integrated sampling enrichment equipment and an immunomagnetic bead incubation technology. Collecting and incubating microorganisms in the aerosol by using integrated sampling and enriching equipment equipped with immunomagnetic beads; intercepting a large-size magnetic bead-microorganism combination body by using a nano-channel membrane; and an electrochemical sensing platform is used for representing sampling and capturing effects. According to the invention, efficient enrichment of aerosol microorganisms is realized through cooperation of immunomagnetic beads and integrated sampling enrichment equipment. According to the integrated sampling and enriching equipment disclosed by the invention, a microorganism pretreatment module and a detection module are highly integrated, rapid separation and high-sensitivity detection of microorganisms are considered, and in addition, the integrated sampling equipment is