Danaoqing'e tablet characteristic spectrum construction and quality control method

• Main Authors: SUN NAN, HE JIAN, TANG XIAODONG, WANG LIANG, WANG YONG, MA PENGGANG
• Format: Patent
• Language: Chinese; English
• Published: 14.07.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a Danaoqing'e tablet characteristic chromatogram construction and quality control method, and the Danaoqing'e tablet characteristic chromatogram construction method comprises the following steps: carrying out gradient elution on a test solution under the following chromatographic conditions; a chromatographic column: octadecylsilane chemically bonded silica is used as a filler, and the column temperature is 40-45 DEG C; a mobile phase A is a 0.2% formic acid aqueous solution, and a mobile phase B is methanol; the preparation method of the test solution comprises the following steps: taking a test with the mass of M1, precisely weighing, adding a solvent for ultrasonic treatment, cooling, adding the solvent to fix the volume to the volume V1, uniformly shaking, centrifuging to take a supernatant or filtering to take a subsequent filtrate, thereby obtaining the test solution. According to the method disclosed by the invention, through the optimized matching of each chromatographic condit