Porcine epidemic diarrhea virus S1 protein trimer gene and application thereof

• Main Authors: SHEN YUAN, XIANG CONGYING, LIU HANPING, CHEN GUIHUA, OU RENFANG, ZHANG DONGDONG
• Format: Patent
• Language: Chinese; English
• Published: 14.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a porcine epidemic diarrhea virus S1 protein trimer gene and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The vaccine prepared by emulsifying the optimized gene expressed porcine epidemic diarrhea virus S1 protein tripolymer and an adjuvant is good in safety and high in antibody titer, the immune efficacy and the immune duration are both superior to those of an inactivated vaccine, and the vaccine can achieve an ideal immune effect only through single immunization and has practical application value. 本发明涉及一种猪流行性腹泻病毒S1蛋白三聚体基因及其应用，其中基因的核苷酸序列如SEQ ID NO.1所示。本发明通过优化后的基因表达的猪流行性腹泻病毒S1蛋白三聚体，与佐剂乳化，制成的疫苗安全性好，抗体效价高，免疫效力和免疫持续期，均优于灭活疫苗，且该疫苗只需单次免疫即可达到理想的免疫效果，具备实际应用价值。