Recombinant bacterium for exogenous expression of alanine racemase and application of recombinant bacterium

• Main Authors: LIU LEI, MA XIANGLIANG, LIU SHUPENG, ZHANG DAWEI
• Format: Patent
• Language: Chinese; English
• Published: 11.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a recombinant bacterium for exogenous expression of alanine racemase and application thereof, the recombinant bacterium for exogenous expression of alanine racemase is bacillus subtilis for expression of alanine racemase, and the amino acid sequence of alanine racemase is as shown in SEQ ID NO: 2; the method comprises the following steps: constructing a recombinant expression vector containing an alanine racemase coding gene, and converting the recombinant expression vector into bacillus subtilis, wherein the alanine racemase coding gene has a nucleotide sequence as shown in SEQ ID NO: 1; the recombinant strain can be used for preparing fermentation liquor containing alanine racemase. Fermentation liquor of recombinant bacteria subjected to induced expression or fermentation liquor containing alanine racemase can be used for catalyzing L-alanine to synthesize DL-alanine, the L-alanine conversion rate is high, byproducts are few, the fermentation time is short, the cost is low, and the m