Method for simultaneously separating six phenolic substances in elm leaves, peels and fruits and application

• Main Authors: LIU XIN, LIN NAN, GUO PENG, WEI DONGWEI, SHANG FUDE
• Format: Patent
• Language: Chinese; English
• Published: 07.07.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of plant component analysis, and particularly relates to a method for simultaneously separating six phenolic substances in elm leaves, peels and fruits and application. The method comprises the following steps: gradient elution separation is carried out by using a solid-liquid separation method, a mobile phase A comprises 5 mmol of ammonium acetate and 0.1% formic acid, and a mobile phase B is methanol; the gradient elution procedure comprises 0 to 1 min, 5% B, 1 min to 3 min, 30% B, 3 min to 3.1 min, 50% B, 3.1 min to 4.5 min, 95% B, 4.5 min to 7 min, 95% B, 7 min to 8 min and 5% B. RSD values of the precision of the six phenolic substances detected based on the method are all 3% or below, and the method has good precision; the repetitive RSD value has good repeatability; the recovery rate is 86.17% or above, and the good recovery rate is achieved; the analysis can be completed within 10 minutes, and the analysis speed is high. 本发明属于植物组分分析技术领域，具体涉及一种同时分离榆树叶皮果中六种酚类