Peanut low-temperature-resistant gene AhCOLD1 and cloning, expression analysis and application method thereof

• Main Authors: CHEN ZHIDE, LIU YONGHUI, SHEN YUE, LIANG MAN, ZHANG XUYAO, SHEN YI
• Format: Patent
• Language: Chinese; English
• Published: 07.07.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a peanut low-temperature-resistant gene AhCOLD1 and cloning, expression analysis and application methods thereof, and relates to the technical field of gene engineering, the peanut low-temperature-resistant gene AhCOLD1 comprises a nucleotide sequence and a coding amino acid sequence; the cloning method of the peanut low-temperature-resistant gene AhCOLD1 comprises a sampling step, a reverse transcription step and an amplification method. According to the expression analysis method, the expression of AhCOLD1 in different germplasms in a low-temperature environment is analyzed by using fluorescent quantitative PCR (Polymerase Chain Reaction); when the peanut low-temperature-resistant gene AhCOLD1 is applied to arabidopsis thaliana, the germination and growth capabilities of arabidopsis thaliana at low temperature can be improved, and a theoretical basis is provided for low-temperature-resistant mechanism research of peanuts and low-temperature-resistant breeding of peanuts. 本发明提供了一种花生耐低温