Application of SnRK1 beta subunit in rice resistance to magnaporthe oryzae and xanthomonas oryzae

• Main Authors: WANG XINGBIN, PENG YOULIANG, LU XUNLI, YUAN GUIXIN
• Format: Patent
• Language: Chinese; English
• Published: 07.07.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a rice SnRK1 beta subunit protein related to plant disease resistance as well as a coding gene and application of the rice SnRK1 beta subunit protein. The SnRK1 beta protein provided by the invention is a protein as shown in a sequence 2, 4 or 6, and the encoding gene of the SnRK1 beta subunit protein is a DNA molecule as shown in a sequence 1, 3 or 5. Through the knockout of the SnRK1 beta subunit, the resistance to rice blast and bacterial blight is enhanced. After treatment of a pathogen-related molecular pattern (Chitin), up-regulated expression of pathogen-related proteins is caused, ROS level accumulation is caused, and an MAPK pathway is activated. In addition, the deletion of the SnRK1 beta 1A gene does not influence the main agronomic traits of the rice, which shows that the SnRK1 beta gene has better application potential in enhancing the disease resistance of crops. According to the invention, molecular breeding is carried out by regulating the expression level of the SnRK1b