Preparation and application of nucleic acid extraction material of chitosan modified attapulgite

• Main Authors: YU HANG, YAO WEIRONG, XING JIALI, DONG HAN, CHENG YULIANG, WANG MOFAN, QIAN HE, GUO YAHUI, LUO XIAOHU, XIE YUNFEI
• Format: Patent
• Language: Chinese; English
• Published: 07.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

The invention discloses preparation and application of a nucleic acid extraction material of chitosan modified attapulgite, and belongs to the technical field of biology. The method comprises the following specific steps: S1, acid activation modification: stirring attapulgite powder and an HCl aqueous solution to react, filtering, washing a solid, drying, and then grinding and sieving to obtain acid activated attapulgite; s2, chitosan modification: adding chitosan and the acid activated attapulgite into hydrochloric acid, stirring, adjusting the pH value to be neutral, drying the solution, grinding and drying to obtain a solid, and sieving to obtain the chitosan modified attapulgite. The chitosan is utilized to modify the attapulgite, the physical and chemical properties of the attapulgite are optimized, the nucleic acid adsorption performance of the attapulgite is greatly improved, and meanwhile it is ensured that the cost is low. 本发明公开了一种壳聚糖改性凹凸棒土的核酸提取材料的制备及应用，属于生物技术领域。该方法具体步骤如下：S1、酸活化改性：将凹凸棒土粉末与HCl水溶液搅拌反应，