Chitosan nano-liposome freeze-dried preparation and preparation method thereof

• Main Authors: YUAN ZHONGWEN, DUAN YINGYING, FENG SENLING, HUANG HONGLIANG
• Format: Patent
• Language: Chinese; English
• Published: 07.07.2023
• Subjects: HUMAN NECESSITIES; HYGIENE; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEDICAL OR VETERINARY SCIENCE; NANOTECHNOLOGY; PERFORMING OPERATIONS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; TRANSPORTING

The invention provides a chrysoidine nano-liposome freeze-drying preparation and a preparation method thereof, and the preparation method comprises the following steps: S1, adding chrysoidine and a phospholipid carrier into an organic solvent, and uniformly mixing to obtain an organic phase; s2, adding cholesterol sodium sulfate into water, and ultrasonically dispersing uniformly to obtain a water phase; s3, pouring the organic phase obtained in the step S1 into the water phase obtained in the step S2, and performing probe ultrasonic treatment for 2-3 minutes under an ice bath condition to form O/W type primary emulsion; s4, magnetically stirring the O/W type primary emulsion obtained in the step S3 under the water bath condition of 45 DEG C for 15-20 minutes, and then performing ultrasonic treatment on a probe for 8-10 seconds to obtain an emulsion; and S5, filtering the emulsion obtained in the step S4 through a microfiltration membrane, centrifuging for 5-15 minutes, taking the supernatant to obtain a lipo