Culture medium for inducing phytophthora tropicalis to produce spores as well as preparation method and application of culture medium

• Main Authors: WEN SIWEI, GOU YAFENG, TIAN TIAN, MENG QIANQIAN, XUE CHAO, GAO SHENGFENG, LIU SHICHAO, SUN SHIWEI
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of microorganisms, in particular to a culture medium for inducing phytophthora tropicalis to produce spores, a preparation method and application. According to the culture medium and the method for inducing the phytophthora tropicalis to generate the zoospores, the yield of the zoospores reaches 1.32 * 10 < 6 >/ml and is far higher than that of similar culture mediums. The cucumber, the pepper leaves and the vitamin C which serve as main raw materials are low in price, the preparation method is simple and easy to implement, and the obtained zoospore suspension is pure and sterile and is very suitable for mechanism research and prevention and control technology research and development of phytophthora tropicalis. 本发明涉及微生物领域，特别涉及诱导热带疫霉产孢的培养基、制备方法及应用。本发明提供的培养基及其诱导热带疫霉菌产生游动孢子的方法，游动孢子产量达1.32×106个/ml，远高于同类培养基。本发明主要原料黄瓜、胡椒叶和维生素C、价格便宜，配制方法简便易行，获得的游动孢子悬浮液纯净无菌，非常适用于热带疫霉机理研究和防控技术研发。