Detection method, probe composition and kit for intraocular fluid Q hot rickettsia

• Main Authors: TAO YONG, LU CHENGHUI, DENG YONGJIE
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a detection method of intraocular fluid Q hot rickettsia, a probe composition and a kit, and relates to the technical field of molecular diagnosis. The probe composition comprises a Cb-IS1111A probe group and/or a Cb-com1 probe group, wherein the Cb-IS1111A probe group is composed of a primer Cb-IS1111A-F1, a primer Cb-IS1111A-R2 and a probe Cb-IS1111A-P1, and the probe Cb-com1 probe group is composed of a primer Cb-IS1111A-F1, a primer Cb-IS1111A-R2 and a probe Cb-IS1111A-P1. And the Cb-com1 probe group is composed of a primer Cb-com1-F2, a primer Cb-com1-R2 and a probe Cb-com1-P1, and the primer Cb-com1-F2, the primer Cb-com1-R2 and the probe Cb-com1-P1 are used for detecting the fluorescence intensity of the probe. The probe composition has the advantages of good target gene amplification specificity and high sensitivity, and solves the problems of low detection speed, tedious and complicated diagnosis operation and pathogen infection risk in the traditional detection mode of Q hot r